Bradford Reagent, ready-to-use, is a convenient 1X concentrated dye reagent for rapid and accurate estimation of total protein concentration in solution. The Bradford protein assay relies on the formation of complexes between Coomassie Brilliant Blue G-250 dye and proteins in solution.
The binding of the dye to the protein causes a shift in the absorption maximum of the dye from 465 nm to 595 nm.Unknown protein concentrations are determined by reference of their absorptions to a series of standard protein dilutions. The quantification procedure is simple and rapid. The dye binding process is complete in approximately 5 min with color stability for 1 hour.
The working range is 1-2000 µg/ml of protein. Depending on the protein concentration, standard or microassay procedures can be used. The assay can be performed in test tubes or microplates.